Fig. 7.

Both human C-MSC and C-MSCγ xenografts induce SCID mouse calvarial bone regeneration. Cultured human C-MSC or C-MSCγ (50) in vitro were transplanted into a NOD/SCID mouse calvarial defect 1.6 mm in diameter. a Micro-CT images of bone regeneration by C-MSC or C-MSCγ (50). b The calvarial defect area was scanned by micro-CT on day 28. The point indicates the individual data and the bar shows the mean (n = 4/group). ^* p < 0.05: values differ significantly (U test). c All mice were sacrificed 28 days after cell transplantation and the calvarial bones were fixed. Coronal sections were obtained and stained with H&E. Lower magnification (×100) is indicated. Bar = 200 μm. huC-MSC xenotransplantation of human clumps of a mesenchymal stem cell/extracellular matrix complex (C-MSC) cultured in growth medium for 3 days, huC-MSCγ (50) xenotransplantation of human C-MSC treated with 50 ng/mL IFN-γ for 24 h before the end of the culture period