Table 1.

Sense primers and antisense primers for real-time PCR

Target gene		Primer sequence
IDO	Forward	5′-CAAAGGTCATGGAGATGTCC-3′
	Reverse	5′-CCACCAATAGAGAGACCAGG-3′
ALPase	Forward	5′-GCGGTGAACGAGAGAATG-3′
	Reverse	5′-CGTAGTTCTGCTCGTGCAC-3′
BMP-2	Forward	5′-CTGTATCGCAGGCACTCA-3′
	Reverse	5′-CTCCGTGGGGATAGAACTT-3′
OC	Forward	5′-GCAGCGAGGTAGTGAAGAGAC-3′
	Reverse	5′-GGTCAGCCAACTCGTCACAG-3′
OPN	Forward	5′-GATGGCCGAGGTGATAGTGT-3′
	Reverse	5′-CCATTCAACTCCTCGCTTTC-3′
GAPDH	Forward	5′-AACGTGTCAGTGGTGGACCTG-3′
	Reverse	5′-AGTGGGTGTCGCTGTTGAAGT-3′

ALPase alkaline phosphatase, BMP bone morphogenic protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IDO indoleamine 2,3-dioxygenease, OC osteocalcin, OPN osteopontin