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A: effect of LPS on activity of CK2 in NCM460 cells. Cells were treated with LPS (50 µg/ml; 4 h). Activity of CK2 was determined on cell lysate as described in Methods. B: effect of specific inhibitors of CK2 on biotin uptake by NCM460 cells. Cells were treated with the CK2 inhibitors (TBB and TBCA, 30 µM) for 1 h, followed by determination of biotin uptake (see Methods). Data are means ± SE of at least 3 independent experiments (*P < 0.05, **P < 0.01).
