Fig. 5.

Trpv4 mRNA expression in renal cells. A: Trpv4 mRNA was detected by RT-PCR in a mIMCD-3 RNA sample treated with reverse transcriptase (RT) but not in an untreated sample. PCR products were subjected to electrophoresis on a 4% agarose gel stained with ethidium bromide. The 79-bp PCR product migrated a bit more slowly than the 80-bp marker, but its identity was confirmed with sequencing. Trpv4 mRNA was also detected in RT reactions from 3 additional, independent mIMCD-3 cultures (data not shown). B: as measured by RT-qPCR, expression of Trpv4 mRNA was similar in ciliated (176-5) and nonciliated (176-5Δ) renal cells, regardless of which reference gene was used: succinate dehydrogenase complex flavoprotein subunit A (Sdha) or B2m (P > 0.5, t-test). For B, n = 3 experiments.