Table 2.
Genes upregulated in mIMCD-3 cells by exposure to 500 mOsm/kg vs. isosmolal conditions
| Gene | Fold Change |
|---|---|
| Cultured on tissue culture-treated plastic | |
| Akr1b3 | 65.27 |
| Aqp1 | 26.04 |
| Plat | 18.54 |
| Pax2 | 11.45 |
| Sgk1 | 10.23 |
| Hspa4l | 10.19 |
| Slc5a3 | 9.81 |
| Hspa1a | 6.72 |
| Cryab | 5.71 |
| Adm | 4.83 |
| Dusp1 | 3.94 |
| Atp1b1 | 2.84 |
| Vegfa | 2.83 |
| Slc14a2 | 2.59 |
| Atp1a1 | 2.56 |
| Slc2a1 | 2.36 |
| Nfkbia | 2.25 |
| Slc6a6 | 2.25 |
| Tnf | 2.21 |
| Slc38a2 | 1.76 |
| Cd9 | 1.53 |
| Cultured on Transwell tissue culture-treated semipermeable polycarbonate membrane | |
| Aqp1 | 4.65 |
| Akr1b3 | 4.56 |
| Atp1b1 | 1.99 |
| Hspa1a | 1.89 |
| Cryab | 1.84 |
| Plat | 1.74 |
| Slc5a3 | 1.73 |
| Dusp1 | 1.72 |
| Pax2 | 1.56 |
| Slc38a2 | 1.56 |
Hyperosmolal stress (500 mOsm/kg) was achieved by supplementation of culture medium with NaCl. Genes shown in boldface are common between cells cultured on plastic and permeable supports. Each condition was repeated 3 times, and results were determined on the basis of the average of these 3 replicates. Change ≥1.5-fold was considered an increase.