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. 2016 Nov 9;312(4):F682–F688. doi: 10.1152/ajprenal.00420.2016

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Fig. 1. — Distal convoluted tubule (DCT) preparation for the patch-clamp recording. A: tubule image showing the location of DCT1, DCT2, connecting tubule (CNT), and cortical collecting duct (CCD). B: tubule image showing the split-open DCT2 and CNT. C: image showing a patch-clamp pipette (Pip) was placed in the apical membrane of a split open DCT2/CNT. The pipette position pointed by an arrow is also indicated in A. D: images of isolated DCT/CNT from wild-type (WT) and TgWnk4^PhAII mouse. Note that DCT/CNT tubule from TgWnk4^PHAII mouse is hypertrophic in comparison to WT.