Fig. 7.

Effect of kidney regeneration on ren expression in head kidney vs. tail and trunk regions. To determine any involvement of renin in kidney regeneration, ren mRNA was assessed in separate regions of the regenerating kidney. Differences in mRNA transcripts were tested between the head kidney and trunk and tail region. Regenerating kidneys were selected on the basis of the upregulation of the nephron progenitor marker, Wilm's tumor. Renal damage and the subsequent regenerative response were induced by 75 mg/kg ip injected gentamicin. Expression of mRNA was analyzed 9 days postinjection (dpi). A and B: both homologs of Wilm's tumor are increased at 9 dpi, but differences between regions are not significant. C: the tail and trunk kidney region has significantly more ren mRNA than the head kidney. Slight increases in ren mRNA with regeneration were not significant at 9 dpi. P value summary: ns, P ≥ 0.123; **P ≤ 0.002; and ***P ≤ 0.001.