Table 3.

Did genetic deletion of Calhm1, P2X2+P2X3, SGLT1, or Sur1 alter plasma insulin or blood glucose response to oral stimulation with glucose (experiment 6)?

Signaling protein	Blood Measurement	Source of Variation	F Ratio	df	P Value
Calhm1	Plasma insulin	Time	15.0	4,52	<0.001
		Genotype	<0.1	1,13	0.79
		Interaction	0.8	4,52	0.50
	Blood glucose	Time	25.7	4,52	<0.0001
		Genotype	1.7	1,13	0.21
		Interaction	0.4	4,52	0.82
P2X2+P2X3	Plasma insulin	Time	12.4	4,52	<0.001
		Genotype	1.4	1,13	0.26
		Interaction	0.5	4,52	0.72
	Blood glucose	Time	32.9	4,52	<0.001
		Genotype	1.4	1,13	0.26
		Interaction	5.0	4,52	<0.002
SGLT1	Plasma insulin	Time	10.1	4,72	<0.001
		Genotype	0.7	1,18	0.43
		Interaction	0.9	4,72	0.44
	Blood glucose	Time	14.6	4,72	<0.001
		Genotype	25.3	1,18	<0.001
		Interaction	10.1	4,72	<0.001
Sur1	Plasma insulin	Time	8.7	4,84	<0.001
		Genotype	3.7	1,21	0.07
		Interaction	4.8	4,84	<0.002
	Blood glucose	Time	68.3	4,84	<0.001
		Genotype	82.2	1,21	<0.001
		Interaction	23.3	4,84	<0.001

Actual data are presented in Fig. 9. We performed mixed-model ANOVAs, separately for each transduction protein and blood measurement. In each case, time was a within factor and genotype a between factor.