Fig. 5.

In vitro glucose- and leucine-stimulated insulin secretion is equivalent in isolated anemic and control fetal islets. Pancreatic islets were isolated from anemic (open bars; n = 8) and control (solid bars; n = 8) fetuses. In vitro insulin secretion was assayed in 1-h static incubations with Krebs-Ringer bicarbonate buffer and additional glucose and leucine as indicated. Although glucose and leucine stimulated insulin secretion, there were no differences between anemic and control islets. Data are presented as means ± SE, and statistics were obtained through mixed-model ANOVA. Incubations with different letters were significantly different from each other (P < 0.0005).