Table 1.
Oligonucleotide sequences used for molecular cloning and real-time RT-PCR detection of Ntcp/Slc10a1, Bsep (Abcb11), ActB (β-actin) genes from skate and mouse
| Name | Sequence (5′→3′) | Purpose | Gene |
|---|---|---|---|
| skSlc10a1-F9 | CGATGATCTCCTTGGGCTGCACC | Cloning | skate Slc10a1 |
| skSlc10a1-R10 | GAGCACCAATCTCCTCTCTGCAG | Cloning | skate Slc10a1 |
| skSlc10a1-F25 | GCATGGATCCTGGAGGGCATTAGCTTCTTGGCT | Cloning | skate Slc10a1 |
| skSlc10a1-R26 | AATGGGCATCAGTGCTGTGGTGA | Cloning | skate Slc10a1 |
| skSlc10a1-R29 | TCACCTTCTGCTTCGATAGATCAC | Cloning | skate Slc10a1 |
| skSlc10a1-F11 | GGACACTTTTCTCTCCTCACCTAATT | Cloning and Q-PCR | skate Slc10a1 |
| skSlc10a1-R13 | CGCAAGAGCATATCCTGAAGTGTA | Cloning and Q-PCR | skate Slc10a1 |
| skSlc10a1-P12 | Fam-TCACCACAGCACTGATGCCCATTATTG-BHQ | Q-PCR | skate Slc10a1 |
| skBsep-3854F | TTGCCATCGCACGAGCCAT | Q-PCR | skate Bsep |
| skBsep-3920R | AGCGCAGACGTGGCCTCAT | Q-PCR | skate Bsep |
| skBsep-3875P | Fam-TCCGTGACCCAAAGATCCTGCTCCTG-BHQ | Q-PCR | skate Bsep |
| skActB-564F | GAGTCACACTGTGCCCATCTACG | Q-PCR | skate ActB |
| skActB-630R | AGCCAGGTCCAGACGCAGG | Q-PCR | skate ActB |
| skActB-588P | Hex-AGGCTACGCCTTGCCACACGCC-BHQ | Q-PCR | skate ActB |
| mBsep TaqMan PP | Purchased from Life Technologies, Cat no. Mm00445168_m1 | Q-PCR | mouse Bsep |
| mNtcp TaqMan PP | Purchased from Life Technologies, Cat no. Mm00441421_m1 | Q-PCR | mouse Ntcp |
| mActB TaqMan PP | Purchased from Life Technologies, Cat no. Mm00607939_s1 | Q-PCR | mouse ActB |
Q-PCR, quantitative PCR; sk, skate; m, mouse.