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. 2017 Jan 27;312(4):H791–H799. doi: 10.1152/ajpheart.00952.2015

Fig. 2.

Fig. 2.

Cystathionine γ-lyase (CSE) promoter activity, CSE mRNA expression, and NFAT transcriptional activity in rat aortic endothelial cells (RAEC). A: cells expressing a full-length (CSE-1) or truncated version (CSE-10) of the CSE promoter upstream of luciferase were treated with vehicle (ethanol) or cyclosporine A (CsA; 1 µM) for 48 h. A Renilla construct was used to account for differences in transfection efficiencies, and data are expressed as the ratio of relative luciferase units (RLU) to relative Renilla units (RRU). B: CSE mRNA expression in RAEC treated with vehicle or CsA for 48 h using cyclophillin B or β-actin as endogenous control. C: cyclooxygenase 2 (COX2) mRNA expression in RAEC treated with vehicle or CsA for 48 h using cyclophillin B as endogenous control. P < 0.05 vs. control or vehicle (*) or CSE-1 (#) and ***P < 0.0001; n = 5–6 batches of RAEC from passage 2 to 5.