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. 2017 Jan 27;312(4):H681–H690. doi: 10.1152/ajpheart.00469.2016

Fig. 5.

Fig. 5.

Site-specific immunoblotting for cMyBP-C phosphorylation. Left: representative images from Western blots using cMyBP-C phospho-specific antibodies against Ser-273 (A), Ser-282 (B), and Ser-302 (C) in whole heart homogenates from NTg-S, NTg-T, Tg-S, and Tg-T mice. STD is molecular weight standard. Right: quantification of cMyBP-C phosphorylation levels at: Ser-273 (A); Ser-282 (B), and Ser-302 (C). Data are represented as means ± SE; n = 6 hearts/group. Statistically significant result based on Tukey’s post hoc test: *P < 0.05. All samples were derived and processed at the same time and in parallel. Black lines demarcate spliced regions of interest.