Fig. 4.

Activation of regulatory T cells (Tregs) and other CD4⁺ T cells in mice vaccinated with P101 peptide. A: peritoneal cavity and mediastinal lymph nodes were harvested from Nur77^GFP mice immunized with P101 or adjuvant only and analyzed by multicolor flow cytometry at 24 hrs. The analyzed populations were gated on live singlet TCRβ⁺CD4⁺ T cells. B: representative plots of Nur77 (by GFP) and FoxP3 (by PerCP-Cy5.5 labeled antibody) expression on peritoneal CD4⁺ T cells (top) and mediastinal lymph node CD4⁺ T cells (bottom). C: histogram of Nur77-GFP expression among FoxP3⁺CD4⁺ T cell from peritoneal cavity (top) and mediastinal lymph node (bottom). Each plot is representative of 4 independent experiments. Percentage of Nur77⁺ cells (D) and FoxP3⁺Nur77⁺ cells (E) among peritoneal CD4⁺ T cells. n = 5 in each group. **P < 0.01. Data are means ± SE. Significance was assessed by Mann-Whitney test.