Cardiac mediator subunit 1 (Med1) expression is dynamic during development and disease. A: immunoblotting of Med1 and Gapdh in mouse heart tissues from time periods embroynic day (E)18.5, postnatal day (P)1, P3, P7, P14, P21, 12 wk, and 1 yr. Left: representative immunoblot. Right: quantitative analysis (right panel). (n = 4/time period). B: immunoblotting of Med1 and α-actinin in mouse ventricular tissue from mice that underwent a sham operation [sham: heart weight (HW; mg): 119 ± 6.8: ejection fraction (EF; %): 72 ± 5.2], a transaortic constriction where there was a compensated hypertrophic cardiomyopathy (HCM: HW: 196 ± 5.6; EF:50 ± 6.1), and a transaortic constriction where the hearts developed a dilated cardiomyopathy (DCM: HW:261 ± 20; EF: 19 ± 4.9). Top: representative immunoblot. Bottom: quantitative analysis. *P < 0.05 vs. sham control; n = 4/group. C: immunoblotting of Med1 and α-Actinin in human ventricular tissue samples from patients with nonfailing hearts (NF) or dilated cardiomyopathy (DCM). Top: representative immunoblot. Bottom: quantitative analysis. *P < 0.05 vs. NF control; n = 3/group.