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. 2017 Feb 3;312(4):H768–H780. doi: 10.1152/ajpheart.00728.2016

Fig. 5.

Fig. 5.

Med1 deletion leads to changes in metabolic gene and transcriptional regulators in the heart. Data were acquired from 3-wk-old Med1cKO hearts and Med1cKO-MCM hearts 21–28 days after the start of tamoxifen administration. A–D: RT-qPCR validation of mRNA expression for genes encoding enzymes involved in cardiac metabolism (A and B) or regulatory transcription factors (C and D) in Med1cKO and Med1cKO-MCM ventricular samples. Data are means ± SE; n = 5–6. *P < 0.05 vs. Med1fl/fl controls. Fatty acids uptake genes: Fatp and Fatpb3. Fatty acid transport Cpt1a, Cpt1b, and Cpt2. β-Oxidation genes: Acadm and Acadvl. Oxidative Phosphorylation genes: Cs and Ckmt2. Transcriptional regulators: Essra, Ppara, Pargc1a, and Ppargc1b. E and F: immunoblotting of transcriptional regulatory proteins Pgc1α, Pparα and Errα in Med1cKO and Med1cKO-MCM ventricular samples. Top: representative immunoblot. Bottom: quantitative analysis. Data are means ± SE (n = 6/group); *P < 0.05 vs. Med1fl/fl controls.