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. 2017 Feb 10;312(4):H711–H720. doi: 10.1152/ajpheart.00724.2016

Fig. 6.

Fig. 6.

A and B: after 48 h of transfection with nontargeted siRNA (Consi) or HDAC6-specific siRNA (HDAC6si) using RNAimax (Invitrogen), HAEC were incubated in medium with or without OxLDL for 24 h, and cell lysates were subjected to Western blotting with CSEγ, acetylated α-tubulin, total α-tubulin, and acetylated α-tubulin and HDAC6 antibodies. C and D: H2S in cell lysates and nitrite levels in culture medium. *P < 0.05 vs. respective controls. Western blots are representative of 3 immunoblots. Values are means ± SE; n = 6.