Figure 1.

ThT fluorescence of γS-WT and γS-G18V prepared at variable pH and two temperatures. (A) For γS-WT at 25°C, only background fluorescence is observed. For γS-G18V, elevated ThT fluorescence is observed at both low (2–3) and high (8–9) pH. Only heated samples (37°C) at pH 2 and 9 exhibit elevated fluorescence. (B) At physiological temperature (37°C), elevated ThT fluorescence is seen in γS-WT at pH 2 and 9, while γS-G18V displays significant increases in signal at pH 2, 3, 8, and 9.