Fig. 2. DHPFQ detection of NO in Raw 264.7 cells. (Top) Incubated with DHPFQ (50 μM) for 8 h; (Middle) pretreated with LPS (0.5 μg mL^–1) for 4 h and incubated with DHPFQ (50 μM) for 8 h; (Bottom) pretreated with N ^G-monomethyl-l-arginine (2 mM), a NOS inhibitor, for 1.5 h and incubated with LPS (0.5 μg mL^–1) for 4 h, then DHPFQ (50 μM) for 8 h. Green, DHPFQ; blue, DAPI; red, Lytracker Red. Scale bars are all 10 μm.