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. 2017 Mar 6;23:34–49. doi: 10.2119/molmed.2016.00083

Figure 3.

Figure 3.

Effect of α7 nAChR on body-weight loss, lung fibrogenic and proinflammatory genes during the development of BLM-induced lung fibrosis. (A) Effect α7 nAChR on body-weight loss during the development of BLM-induced lung fibrosis. Chrna7+/+ and Chrna7-/- mice were intratracheally challenged with BLM (1.5 mg/kg) and followed for 14 d. N = 18–20 in each group. *p < 0.05 for Chrna7+/+ versus Chrna7-/- mice receiving intratracheal BLM. Data are mean ± SD. (B–D) Effect of α7 nAChR on lung fibrogenic genes during the development of BLM-induced lung fibrosis. Experimental protocol was the same as Figure 3A. The mice were euthanized at 7 d and the lungs were homogenized for extracting RNA to measure (B) Col1a1, (C) Fsp1 and (D) Fstl1 by qPCR analysis. N = 3–6 in each group. *p < 0.05, **p < 0.01, ***p < 0.001 in each gene comparison for Chrna7+/+ versus Chrna7-/- mice receiving intratracheal BLM. (E–G) Effect of α7 nAChR on lung proinflammatory genes during the development of BLM-induced lung fibrosis. Experimental protocol was the same as Figures 2B–D. The lungs were homogenized for extracting RNA to measure (E) Cxcl2, (F) Mcp1 and (G) Arg1 by qPCR analysis. N = 3–5 in each group. ***p < 0.001 in each gene comparison for Chrna7+/+ versus Chrna7-/- mice receiving intratracheal BLM. Data are mean ± SD.