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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Apr 14;36(6):1164–1173. doi: 10.1161/ATVBAHA.116.307461

Figure 5. Surface mobilization of platelet thiol isomerase is independent of Munc13-4.

Figure 5

A) Immunoblot analysis of platelet lysates from wild type (WT) and Unc13dJinx mice shows lack of Munc13-4 in the latter and similar levels of PDI and ERp57 (GAPDH loading control). B) Flow cytometry measurement of surface P-selectin (CD62P) and PDI in platelets under resting conditions or after stimulation with PAR4-AP in the presence/absence of ADP shows that P-selectin mobilization is significantly impaired in Unc13dJinx platelets but PDI mobilization is greater than WT. ADP significantly increased PDI mobilization as compared to WT or resting Unc13dJinx platelets (*P<0.05, *** P<0.001, one-way ANOVA, followed by Dunnett's test or two-way ANOVA, followed by Bonferroni's test, n=4).