Figure 6. SRY expression in early human testis and differential expression of other genes.

( a) Heatmap showing normalised gene expression values for SRY across the whole sample dataset. ( b) Scatter plot of normalised gene expression of SRY in testis samples between 45 and 74 dpc. Loess method was used to fit a smooth curve between expression values. ( c) Heatmap representing normalised gene expression values for the top 30 differentially up-regulated genes in early testis samples (CS18 and CS19 stages) ( N=4) compared to control samples ( N=6). Genes are ordered according to decreasing log ₂FC values. The intensity of gene expression is indicated by a colour scale: blue for lowest and red for highest expression levels. For all samples shown, P-value≤0.05.