Skip to main content
. 2017 Mar 30;41(5):939–954. doi: 10.1111/acer.13368

Figure 3.

Figure 3

LPS, Poly(I:C), and EtOH activate proinflammatory‐related kinases/transcription factors in microglia‐like BV2. Microglia‐like BV2 were treated with indicated drug for 10 or 30 minutes. Cell lysates were collected for Western blot analysis. (A) Ten minutes of LPS (100 ng/ml) increased p‐ERK1/2 by 29%, p‐p38 by 98%, and p‐NF κB‐p65 by 42%. Thirty minutes of LPS increased p‐ERK1/2 by 68%, p‐p38 by 568%, and p‐NF κB by 80%. (B) Ten minutes of Poly(I:C) (50 μg/ml) increased p‐ERK1/2 by 21%. Thirty minutes of Poly(I:C) increased p‐ERK1/2 by 21%, p‐p38 by 41%, and p‐NF κB by 40%. (C) Ten minutes of EtOH (150 mM) decreased p‐ERK1/2 by 38%. Thirty minutes of EtOH decreased p‐ERK1/2 by 40% and increased p‐IRF3 by 50%. Data are represented as %control (%CON) following β‐actin normalization. n = 3 per group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus CON.