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. 2017 Mar 30;41(5):939–954. doi: 10.1111/acer.13368

Figure 6.

Figure 6

Poly(I:C) and EtOH, but not LPS, activate proinflammatory‐related kinases/transcription factors in neuron‐like SH‐SY5Y. SH‐SY5Y were treated with indicated drug for 10 or 30 minutes. Cell lysates were collected for Western blot analysis. (A) LPS (100 ng/ml) had no detectable effect on any of the kinases/transcription factors examined. (B) Poly(I:C) (50 μg/ml) increased p‐p38 by 88% and p‐IRF3 by 38% by 30 minutes. (C) Ten minutes of EtOH (150 mM) decreased p‐ERK1/2 by 55%, and increased p‐p38 by 49%, p‐IRF3 by 41%, p‐TBK1 by 74%, and p‐NF κB by 56%. Thirty minutes of EtOH decreased p‐ERK1/2 by 49%, and increased p‐p38 by 222%, p‐IRF3 by 125%, p‐TBK1 by 94%, and p‐NF κB by 73%. Data are represented as %CON following β‐actin normalization. n = 3 per group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus CON.