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. 2017 Mar 30;41(5):939–954. doi: 10.1111/acer.13368

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Copyright © 2017 The Authors Alcoholism: Clinical and Experimental Research published by Wiley Periodicals, Inc. on behalf of Research Society on Alcoholism.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Poly(I:C), but not LPS, up‐regulates cytokine expression in neuron‐like SH‐SY5Y. SH‐SY5Y were treated with either LPS (100 ng/ml) or Poly(I:C) (50 μg/ml) for 24 hours followed by mRNA analysis in cell lysates and analysis of HMGB1 release in media using ELISA. (A) LPS had no detectable effect on expression of HMGB1, TNF‐α, IL‐1β, or IL‐6 mRNA. (B) LPS had no effect on HMGB1 release in media. (C) Poly(I:C) increased expression of HMGB1 (125%), TNF‐α (10,426%), IL‐1β (2,860%), and IL‐6 (591%) mRNA. (D) Poly(I:C) had no effect on HMGB1 release in media. Data for mRNA are expressed as %control (%CON), with CON set at 100%. n = 3 per group; n.d. = not detectable; *p < 0.05, **p < 0.01 versus CON.