Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 30;41(5):939–954. doi: 10.1111/acer.13368

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Authors Alcoholism: Clinical and Experimental Research published by Wiley Periodicals, Inc. on behalf of Research Society on Alcoholism.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

EtOH up‐regulates proinflammatory genes in neuron‐like SH‐SY5Y. SH‐SY5Y were treated with EtOH (150 mM) for 24 hours and analyzed for mRNA expression of cytokines, TLRs and TLR‐associated signals, and HMGB1 release in media. (A) EtOH had no detectable effect on HMGB1, TNF‐α, IL‐1β, or IL‐6 mRNA. (B) EtOH increased expression of TLR3 (398%), TLR7 (574%), TLR8 (319%), and RAGE (170%) mRNA. (C) EtOH increased release of HMGB1 in the media by 161%. (D) Concentration curve of 24 hours of EtOH treatment on mRNA expression in SH‐SY5Y. EtOH increased expression of TLR3, TLR7, and RAGE mRNA by 15 mM. Data for mRNA are expressed as %control (%CON), with CON set at 100%. n = 3 per group; n.d. = not detectable; *p < 0.05, **p < 0.01, ***p < 0.001 versus CON.