Table 1.

Primers Used in RT‐PCR Analysis

Gene	Species	Forward (5′‐3′)	Reverse (5′‐3′)
β‐actin	Human	GAT GCA GAA GGA GAT CAC TGC	ATA CTC CTG CTT GCT GAT CCA
TLR2	Human	GGCTTCTCTGTCTTGTGACC	GGGCTTGAACCAGGAAGACG
TLR3	Human	TTG CCT TGT ATC TAC TTT TGG GG	TCA ACA CTG TTA TGT TTG TGG GT
TLR4	Human	CTC TGG GGA GGC ACA TCT TC	CCC AGG TGA GCT GTA GCA TT
TLR7	Human	GATAACAATGTCACAGCCGTCC	GTTCCTGGAGTTTGTTGATGTTC
TLR8	Human	ATG TTC CTT CAG TCG TCA ATG C	TTG CTG CAC TCT GCA ATA ACT
CD14	Human	AGA GGC AGC CGA AGA GTT CAC	GCG CTC CAT GGT CGA TAA GT
MD‐2	Human	GAA GCT CAG AAG CAG TAT TGG GTC	GGT TGG TGT AGG ATG ACA AAC TCC
Myd88	Human	CCGCGCTGGCGGAGGAGATGGAC	GCAGATGAAGGCATCGAAACGCTC
RAGE	Human	CTA CCG AGT CCG TGT CTA CCA	CAT CCA AGT GCC AGC TAA GAG
HMGB1	Human	GGA GAT CCT AAG AAG CCG AGA	CAT GGT CTT CCA CCT CTC TGA
TNF‐α	Human	CCC AGG CAG TCA GAT CAT CTT CT	ATG AGG TAC AGG CCC TCT GAT
IL‐1β	Human	ATG ATG GCT TAT TAC AGT GGC AA	GTCGGAGATTCGTAGCTGGA
IL‐6	Human	ACT CAC CTC TTC AGA ACG AAT TG	CCA TCT TTG GAA GGT TCA GGT TG
β‐actin	Mouse	GTA TGA CTC CAC TCA CGG CAA A	GGT CTC GCT CCT GGA AGA TG
TLR2	Mouse	GCA AAC GCT GTT CTG CTC AG	AGG CGT CTC CCT CTA TTG TAT T
TLR3	Mouse	GTG AGA TAC AAC GTA GCT GAC TG	TCC TGC ATC CAA GAT AGC AAG T
TLR4	Mouse	ATG GCA TGG CTT ACA CCA CC	GAG GCC AAT TTT GTC TCC ACA
TLR7	Mouse	ATG TGG ACA CGG AAG AGA CAA	GGT AAG GGT AAG ATT GGT GGT G
TLR8	Mouse	GAA AAC ATG CCC CCT CAG TCA	CGTCACAAGGATAGCTTCTGGAA
CD14	Mouse	GCC AAA TTG GTC GAA CAA GC	CCA TGG TCG GTA GAT TCT GAA AGT
MD‐2	Mouse	CGC TGC TTT CTC CCA TAT TGA	CCT CAG TCT TAT GCA GGG TTC A
Myd88	Mouse	TCA TGT TCT CCA TAC CCT TGG T	AAA CTG CGA GTG GGG TCA G
RAGE	Mouse	GAA GGC TCT GTG GGT GAG TC	CCG CTT CCT CTG ACT GAT TC
HMGB1	Mouse	CGC GGA GGA AAA TCA ACT AA	TCA TAA CGA GCC TTG TCA GC
TNF‐α	Mouse	GAC CCT CAC ACT CAG ATC ATC TTC T	CCT CCA CTT GGT GGT TTG CT
IL‐1β	Mouse	CTG GTG TGT GAC GTT CCC ATT A	CCG ACA GCA CGA GGC TTT
IL‐6	Mouse	ACA AGT CGG AGG CTT AAT TAC ACA T	TTG CCA TTG CAC AAC TCT TTT C