Table 2.
Summary Basal mRNA Expression of Proinflammatory Genes in Microglia‐Like BV2 and Neuron‐Like SH‐SY5Y
| ddCt | ||
|---|---|---|
| BV2 | SH‐SY5Y | |
| RAGE | 1.03 | 21.4600 |
| HMGB1 | 1.00 | 4.6000 |
| Myd88 | 1.00 | 0.9500 |
| TLR3 | 1.03 | 0.1800 |
| TLR8 | 1.01 | 0.1600 |
| CD14 | 1.01 | 0.0360 |
| TLR2 | 1.01 | 0.0290 |
| TLR7 | 1.01 | 0.0035 |
| TLR4 | 1.01 | 0.0031 |
| TNF‐α | 1.00 | 0.0017 |
| MD‐2 | 1.01 | n.d. |
| IL‐1β | 1.06 | n.d. |
| IL‐6 | 1.02 | n.d. |
Average basal mRNA levels of various immune signaling molecules in BV2 and SH‐SY5Y (n = 3 per group) were analyzed using RT‐PCR and expressed as ddCt, setting BV2 as 1.