Table 3.
Summary of Poly(I:C), LPS, and EtOH Induction of Proinflammatory Gene mRNA in BV2 and SH‐SY5Y
| CON | Poly(I:C) | LPS | EtOH | |
|---|---|---|---|---|
| BV2 | ||||
| TLR2 | 100 ± 10 | 323 ± 70 | 588 ± 69 ** | 170 ± 15 * |
| TLR3 | 100 ± 16 | 158 ± 13 | 133 ± 9.3 | 120 ± 9.1 |
| TLR4 | 100 ± 9.3 | 100 ± 19 | 58 ± 2.2 | 90 ± 8.6 |
| TLR7 | 100 ± 6.0 | 121 ± 24 | 75 ± 3.5 | 158 ± 8.4 ** |
| TLR8 | 100 ± 11 | 49 ± 15 * | 21 ± 2.7 ** | 194 ± 9.5 ** |
| CD14 | 100 ± 7.5 | 154 ± 13 | 242 ± 21 ** | 104 ± 3.7 |
| MD‐2 | 100 ± 11 | 118 ± 31 | 98 ± 13 | 156 ± 4.3 ** |
| Myd88 | 100 ± 1.7 | 113 ± 6.2 | 96 ± 8.5 | 89 ± 7.2 |
| RAGE | 100 ± 16 | 134 ± 7.7 | 98 ± 4.8 | 104 ± 4.4 |
| HMGB1 | 100 ± 4.9 | 104 ± 9.2 | 90 ± 4.3 | 67 ± 9.7 |
| TNF‐α | 100 ± 3.6 | 217 ± 29 * | 440 ± 27 *** | 204 ± 15 ** |
| IL‐1β | 100 ± 9.3 | 366 ± 33 ** | 1,163 ± 45 **** | 304 ± 29 ** |
| IL‐6 | 100 ± 14 | 130 ± 39 | 210 ± 24 * | 10 ± 2.4 ** |
| SH‐SY5Y | ||||
| TLR2 | 100 ± 19 | 230 ± 31 * | 121 ± 30 | 177 ± 23 |
| TLR3 | 100 ± 46 | 920 ± 260 * | 98 ± 35 | 398 ± 80 * |
| TLR4 | 100 ± 23 | 158 ± 12 | 67 ± 18 | 147 ± 14 |
| TLR7 | 100 ± 46 | 234 ± 59 | 153 ± 79 | 575 ± 100 * |
| TLR8 | 100 ± 33 | 103 ± 22 | 121 ± 60 | 320 ± 17 ** |
| CD14 | 100 ± 18 | 146 ± 17 | 61 ± 21 | 127 ± 11 |
| MD‐2 | n.d. | n.d. | n.d. | n.d. |
| Myd88 | 100 ± 13 | 530 ± 30 *** | 79 ± 4.7 | 128 ± 5.9 |
| RAGE | 100 ± 6.6 | 117 ± 8.9 | 61 ± 20 | 170 ± 15 * |
| HMGB1 | 100 ± 4.2 | 125 ± 5.2 * | 84 ± 11 | 105 ± 2.4 |
| TNF‐α | 100 ± 9.5 | 10,426 ± 2,130 ** | 65 ± 17 | 67 ± 13 |
| IL‐1β | n.d. | 2,860 ± 135 | n.d. | n.d. |
| IL‐6 | n.d. | 591 ± 100 | n.d. | n.d. |
Microglia‐like BV2 and neuron‐like SH‐SY5Y were treated with TLR3 agonist Poly(I:C) (50 μg/ml), TLR4 agonist LPS (100 ng/ml), or EtOH (150 mM) for 24 hours. Cell lysates were collected and analyzed for mRNA expression using RT‐PCR. Data for mRNA are expressed as %control (%CON), with CON set at 100%. Data for HMGB1, TNF‐α, IL‐1β, and IL‐6 for LPS and Poly(I:C) treatment in BV2 are also represented in Fig. 4 A,C. Data for HMGB1, TNF‐α, IL‐1β, IL‐6, TLR2, 3, 4, 7, 8, Myd88, Cd14, MD‐2, and RAGE for EtOH treatment in BV2 are also represented in Fig. 5 A,B. Data for HMGB1, TNF‐α, IL‐1β, and IL‐6 for LPS and Poly(I:C) treatment in SH‐SY5Y are also represented in Fig. 7 A,C. Data for HMGB1, TNF‐α, IL‐1β, IL‐6, TLR2, 3, 4, 7, 8, Myd88, Cd14, MD‐2, and RAGE for EtOH treatment in SH‐SY5Y are also represented in Fig. 8 A,B. n.d. = not detectable; n = 3 per group.
Bolded values represent data significantly different versus control. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus CON.