Figure 2. Selinexor combines with immune checkpoint blockade to slow B16F10 melanoma tumor growth.
C57BL/6 mice were injected subcutaneously with B16F10 cells on day 0 and were treated twice per week (Tuesdays and Fridays) with selinexor and immune checkpoint blockade (or appropriate vehicle/isotype control) beginning when tumors became palpable. (a) Selinexor + anti-PD-1. (b) Selinexor + anti-PD-L1. (c) Selinexor + anti-CTLA4. n=6 mice per group. Mean + S.D. *, p<0.05 between selinexor + checkpoint blockade antibody (anti-PD-1, anti-CTLA4, or anti-PD-L1 (100 µg)) and selinexor + isotype control; $, p<0.05 between selinexor + checkpoint blockade antibody (anti-PD-1, anti-CTLA4, or anti-PD-L1 (100 µg)) and vehicle + checkpoint blockade antibody; †, p<0.05 between selinexor + isotype control and vehicle/isotype control; ‡, p<0.05 between selinexor + checkpoint blockade antibody (anti-PD-1, anti-CTLA4, or anti-PD-L1 (100 µg)) and vehicle/isotype control; **, p<0.05 between selinexor + anti-PD-L1 (200 µg) and vehicle + isotype control. Arrows indicate when treatment was initiated.