Figure 4. Selinexor + anti-PD-L1 antibodies induce T cell activation and T_H1 differentiation in melanoma bearing animals.

(a–b) Helper T cells were quantified on the basis of their pattern of chemokine receptor expression. (a) gating strategy: CD4⁺ viable cells (based on forward/side scatter and anti-CD4) were gated based on CCR6 expression: CCR6⁺ cells were defined as T_H17, while CC6⁻ cells were further gated based on expression of CXCR3 (T_H1 cells) and CCR4 (T_H2 cells). (b) Proportion of T_H1 cells among splenic CD4⁺ T cells. (c–e) T cell activation status was assessed on the basis of cell surface phenotype. (c) gating strategy: singly positive CD4⁺ or CD8⁺ viable cells (based on forward/side scatter and anti-CD4 and anti-CD8) were selected and identified as a naïve, early activated/central memory, or effector phenotype based on staining for CD44 and CD62L. (d & e) Proportion of CD4⁺ T cells (d) or CD8⁺ T cells (e) with an early activated/central memory phenotype (CD62L⁺ CD44⁺). n=5–6 mice per group; Mean ± S.D.; *, p<0.05 between indicated groups.