Subject area	Biology
More specific subject area	Label-free quantitative proteomics, Bovine mastitis-associated infectious microalgae, Prototheca. spp.
Type of data	Raw data, table and Excel output files
How data was acquired	LC-MS using an UltiMate 3000 HPLC system (Dionex) connected online to an LTQ-Orbitrap Velos (Thermo Scientific)
Data format	Raw, processed
Experimental factors	a)Cell culture, harvest and protein isolation b)In-solution trypsin digestion and mass spectrometry analysis c)Protein identification and quantitative proteomic analysis
Experimental features	Whole cell proteins were extracted from Prototheca cultured strains cultured until mid-logarithmic phase of growth. For each sample protein concentrations were determined using the Bradford assay (Bio-Rad). Proteins were reduced, alkylated and digested with trypsin in solution. Following LC–MS analysis, protein identification and quantification was performed with MaxQuant software, the label-free quantitation was carried out using Perseus software.
Data source location	Berlin, Germany
Data accessibility	Data available at PRIDE:PXD005305.