Table 1.
Analyte Concentrations (in MEF) For Each Antibody
| Analytea | Concentration (MEF) |
|---|---|
| Level 5 IHControl | |
| HER-2 | |
| For HercepTest, 4B5, CB11b | 1,086,658 |
| For SP3 | 1,023,265 |
| ER | |
| For 1D5 | 934,651 |
| For 6F11, 2-123 | 703,460 |
| For SP1 | 904,259 |
| PR | |
| For 636 | 800,503 |
| For 1294, 1E2 | 1,379,113 |
| For 16 | 1,089,808 |
| Level 4 IHControl (All)c | 77,913 |
| Level 3 IHControl (All) | 8187 |
| Level 2 IHControl (All) | 1331 |
| Level 1 IHControl (All) | 140 |
Abbreviations: MEF, molecular equivalents of fluorochrome; HER-2, human epidermal growth factor receptor type II; ER, estrogen receptor; PR, progesterone receptor.
a
The analytes are defined not only by the molecule to which they bind but also by their antibody epitopes. For this reason, specific antibodies are listed in the “Analyte” column.
b
The HercepTest, 4B5, and CB11 antibodies all bind to the same HER-2 peptide, on the same microbead.
c
The term All refers to all of the HER-2, ER, and PR analytes together on the same microbead.