Figure 2.

NAH suppressed the upregulation of heparanase expression following CLP. (A) The level of heparanase mRNA in the intestinal tissues was determined by real-time PCR. (B) The level of heparanase protein in the serum was measured by ELISA. (C) Intestinal heparanase protein was detected by immunohistochemistry (scale = 50 µm). The figure shows the representative image from each group, and the data are presented as the means ± SD (n=5). Abbreviations: NAH, N-desulfated/re-N-acetylated heparin; CLP, cecal ligation and puncture; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay. *p<0.01, **p<0.001.