Figure 3.

NAH inhibited shedding of syndecan-1 and upregulation of proteases in the intestine of CLP-treated mice. (A) The levels of syndecan-1 in the intestine at 4 hr and 24 hr after CLP were detected by immunohistochemistry (scale = 50 µm). The expressions of (B) MMP-9 and (C) uPA in the intestinal tissues were assayed by Western blotting. This figure presents the representative images, and the data are presented as the means ± SD (n = 5). Abbreviations: NAH, N-desulfated/re-N-acetylated heparin; CLP, cecal ligation and puncture; MMP-9, matrix metalloprotease-9; uPA, urokinase-type plasminogen activator. *p<0.01, **p<0.001.