. 2017 Apr 27;12(4):e0176398. doi: 10.1371/journal.pone.0176398

Table 1. Primers used in this study.

Mutation	Mutagenic oligonucleotide^a
M251L (For)	`5’-TGTGGTTACCGGCGTAGTAGTCCCAAAGTAGGCTCATT-3’`
M251L (Rev)	`5’-AGTGGAATGAGCCTACTTTGGGACTACTACGCCGGTAAC-3’`
N258V (For)	`5’-ACTACTACGCCGGTGTGCACAGCGCTGATCTGGTT-3’`
N258V (Rev)	`5’-GAACCAGATCAGCGCTGTGCACACCGGCGTAG-3’`
V350F (For)	`5’-ACGCGGTTCAGCGCACTTTCGGACCAGGAGGATAC-3’`
V350F (Rev)	`5’-CTTTCCCACTATCCTCCTGGTCCGAAAGTGCGCTGAAC-3’`
I204A (For)	`5’-GCGGAAAACTTTGTAGGTGATGCATACCACGTTGGTTGG-3’`
I204A (Rev)	`5’-CGTGCGTCCAACCAACGTGGTATGCATCACCTACAA-3’`

^a Codons are underlined and italicsindicates bases thatwere changed