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. 2017 Apr 27;12(4):e0176324. doi: 10.1371/journal.pone.0176324

Fig 6. Proliferation and cytokine production of spleen cells.

Fig 6

Single-cell suspensions were prepared from the spleens of individual mice immunized with PBS, TgPrx1-GST, or GST and cultured for 48 h in the presence of Con A, TLA, TgPrx1-GST, GST, or without any stimulator (Medium). (A) Cell proliferation was measured at 48 h. (B) The culture supernatants were assayed for IFN-γ, and IL-4 production with ELISAs. Each bar represents the mean ± standard deviation (PBS; n = 3, TgPrx1 and GST; n = 4). *, statistically significant differences were observed between TgPrx1-GST-immunized group and other groups in same stimulator with two-way ANOVA and Tukey–Kramer post hoc analysis, P < 0.05. #, statistically significant differences were against medium well in same immunized group with two-way ANOVA and Tukey–Kramer post hoc analysis, P < 0.05.