Component	Stock	Final volume for 50 μL reaction
phiX174 plasmid DNA	10 ng/µL	5.0 µL
GoTaq reaction buffer	5x	10.0 µL
dNTP nucleotide mix	10 mM each	1.0 µL
phiX174-F primer	10 μM	2.5 µL
phiX174-R primer	10 μM	2.5 µL
GoTaq DNA Polymerase	(5 U/µL)	0.25 µL
Nuclease-free distilled water	-	28.75 µL