| Component | Stock | Final volume for 30 μL reaction |
| 1st round PCR products | - | 5.0 µL |
| LTR-R primer | 10 μM | 0.9 µL |
| LTR-U primer | 10 μM | 0.9 µL |
| Taqman Probe | 10 μM | 0.3 µL |
| iQ Supermix | 2x | 15.0 µL |
| Nuclease-free distilled water | - | 7.9 µL |
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