Figure 2.

Repeated injections of LAC induced an increase in mGlu2/3 receptor protein levels that outlasted the end of drug treatment. mGlu2/3 protein levels were measured in the dorsal regions of the lumbar spinal cord ipsilateral to CFA injection. Data in (a) and (b) were obtained by the same groups of mice as in Figure 1(a) and (b), respectively. Mice treated with a single injection of saline or LAC were killed after 24 h. Mice treated with saline or LAC for three or seven days were killed pain assessment 1 h following the last injection. Data at seven days from animals used in Figure 1(a) are not shown. Values are means + S.E.M. of four to five and 5–10 determinations in (a) and (b), respectively. *p < 0.05 vs. the corresponding values of mice treated i.p. with saline (one-way ANOVA + Fisher’s LSD; F_(3,23) = 5.884).

Wdt: withdrawal; CFA: complete Freund adjuvant; mGlu2: type-2 metabotropic glutamate.