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. 2015 Jan 7;2:1. doi: 10.1186/s40348-014-0012-0

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© Oglesby et al.; licensee Springer. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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miR-221 is increased in trachea and bronchi and is predicted to target murine ATF6 3’UTR. (A) Relative expression of murine miR-145, miR-221 and miR-494 was determined by qRT-PCR using individual TaqMan murine miRNA assays and normalised to sno412 in the trachea and bronchi of 6-week-old wild-type (n = 9) and βENaC-Tg (n = 6) C57BL/6 mice. Data are represented as mean ± SEM and were compared by t test (*p < 0.01). All qRT-PCR experiments were performed in duplicate and included no-template controls. (B) Predicted binding locations of miR-221 in the full-length 5,422 bp murine ATF6 3′UTR and proposed base pair matches as predicted by TargetScan 6.2.