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. Author manuscript; available in PMC: 2017 Apr 27.
Published in final edited form as: Cancer Res. 2006 May 1;66(9):4602–4609. doi: 10.1158/0008-5472.CAN-05-4196

Figure 4.

Figure 4

Depletion of Id2 leads to up-regulation of E-cadherin and anchorage-independent growth in uveal melanoma cells. A, anchorage-independent colony formation of Mel202 melanoma cells in soft agar, measured as the number of colonies per ×20 field following transfection with an Id2 antisense vector or an empty control vector. DECMA1 indicates that soft agar was incubated with neutralizing anti-E-cadherin antibodies. Columns, mean of triplicate experiments; bars, SE. B, representative bright-field photomicrographs from soft agar assays, showing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide–stained viable tumor cells. Large multicellular spheroids were seen only in cells transfected with the Id2 antisense vector (middle). Bars, 150 μm.