. 2016 Dec 8;28(5):1507–1520. doi: 10.1681/ASN.2016020169

Table 1.

Baseline characterization of Ae1^+/R607H and Ae1^R607H/R607H mice

Characteristics	Units	Ae1^+/+	n	Ae1^+/R607H	n	Ae1^R607H/R607H	n
General
BW	g	24.45±0.39	6	24.11±0.31	10	23.76±0.48	8
Food intake	g/gBW per d	0.17±0.01	6	0.17±0.01	10	0.17±0.01	8
Water intake	ml/gBW per d	0.18±0.02	6	0.18±0.01	10	0.18±0.01	8
Blood
Hct	%	45±0.65	13	45±0.28	12	47±0.80	9
Hb	g/dl	15.1±0.16	9	15.08±0.16	13	15.3±0.36	15
Na⁺	mM	146±0.36	13	147±0.46	12	147±0.47	9
Cl^-	mM	111±0.74	13	115±0.77*	13	118±1.44****	13
K⁺	mM	4.56±0.10	7	4.75±0.07	7	4.45±0.08	5
Ca²⁺	mM	1.25±0.01	13	1.26±0.01	13	1.25±0.01	12
pH		7.32±0.02	13	7.32±0.01	13	7.29±0.02	12
pCO₂	mmHg	43±1.19	12	46±1.16	12	43±0.92	11
HCO₃^–	mM	22±0.55	13	22±0.44	12	21±0.70	12
Urine
Urine output	ml/gBW per d	0.075±0.008	6	0.069±0.005	10	0.065±0.004	8
Urine Osm	mOsm/kgH₂O	3178±383	6	2891±246	10	2670±108	8
Urine pH		6.45±0.04	6	6.95±0.05****	10	7.09±0.05****	8
Na⁺/Creat	mmol/mmol	17±1.19	6	20±0.68	10	21±0.93	8
K⁺/Creat	mmol/mmol	57±11.69	6	63±6.41	10	64±5.99	8
Ca²⁺/Creat	mmol/mmol	0.17±0.02	6	0.15±0.01	10	0.10±0.01*	8

In the basal state, Ae1^+/R607H and Ae1^R607H/R607H mice have no detectable alterations in acid-base parameters, revealing that, as in humans, R607H mutation causes incomplete dRTA with a higher urinary pH. The increase in blood chloride levels is consistent with the presence of a mild hyperchloremic metabolic acidosis. Values are means±SEM. *P<0.05; ****P<0.0001 versus Ae1^+/+ (age 3–5 months, one-way ANOVA). BW, Body weight; gBW, Gram body weight; Hct, Hematocrit; Hb, Hemoglobin; Osm, Osmolarity; mOsm, Milliosmoles; Creat, Creatinine.