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. 2017 Apr 27;12(4):e0176538. doi: 10.1371/journal.pone.0176538

Fig 5. Enhanced Pi absorptive capacity enables ZmPP2AA1 OE to accumulate more phosphorus than the WT and ZmPP2AA1 RNAi plants.

Fig 5

WT and transgenic plants were grown under SP or LP conditions for 15 d. Phosphorus content (A) and phosphorus concentration (B) in WT and transgenic plants. Values represent the means ± SD. Different letters above the bars are used to indicate significant differences (P< 0.05) among means under the same conditions. (C, D) Pi uptake kinetics in WT and transgenic plants under SP and LP conditions. The maize seedlings were subjected to SP (C) and LP (D) conditions for 15 d and were then transferred to Pi starvation conditions without P supplementation for 24 h. The P-depleted plants were transferred to the initial nutrient solution supplemented with 50 μM Pi for the depletion experiment. Pi uptake was measured as the Pi removed from the nutrient solution over time. The points represent observed values. The curve was fitted using an expression based on Michaelis-Menten kinetics.