Skip to main content
. 2017 Apr 27;12(4):e0176243. doi: 10.1371/journal.pone.0176243

Fig 2. Diurnal expression of miR-21 in murine hearts and lungs.

Fig 2

Analysis of cardiac (A) or lung (B) mir-21 and Per2 levels from wildtype mice at Zeitgeber Time (ZT) 3 or ZT15. Total RNA was isolated from murine heart or lung tissue using Qiazol Reagent and separated into mRNA and miRNA components following manufactures instructions (SA-Biosciences, Qiagen). cDNA from miRNA was generated using miScript RT II kits (Qiagen) and transcript levels were determined by quantitative real-time RT-PCR (iCycler; Bio-Rad Laboratories Inc.; mean±SD, n = 3, p<0.05).