Table 1. Results of generalized linear mixed-effects model analysis in Experiment 1 showing the effects of time and water temperature on eDNA concentration.

response variable	explanatory variable	coefficient	standard error
log (ayu sweetfish eDNA)
	elapsed time (h)	–0.024	0.004
	elapsed time (h): water temperature 10°C	0 a
	elapsed time (h): water temperature 20°C	–0.058 b	0.005
	elapsed time (h): water temperature 30°C	–0.091 c	0.005
	Intercept	12.024	0.06
log (common carp eDNA)
	elapsed time (h)	–0.027	0.005
	elapsed time (h): water temperature 10°C	0 a
	elapsed time (h): water temperature 20°C	–0.045 b	0.006
	elapsed time (h): water temperature 30°C	–0.079 b	0.008
	Intercept	7.533	0.07

All explanatory variables were significant at p = 0.05. The differences among coefficients of the interaction terms of elapsed time and water temperature were compared using the 95% confidence interval for the coefficients. The same superscript letters associated with the coefficients indicate statistical equivalence at p = 0.05 in each species. Note that the difference between 20°C and 30°C was marginally significant for common carp (p = 0.07).