Fig 5. Case 3.

Trabeculectomy sample from the left eye. A: Light micrograph. B-G: Transmission electron microscopy. A: Schlemm’s canal (SC) has an open lumen. The corneoscleral trabecular meshwork (CTM) is constituted by quite compact trabecular beams. In the uveal trabecular meshwork (UTM) the intertrabecular spaces (b) are evident. B: The image shows a part of Schlemm’s canal (SC) and the juxtacanalicular tissue. In the SC, only debris of necrotic endothelial cells (N) is visible on a thick basal membrane (bm). In the juxtacanalicular tissue, alternate layers of elastic tissue (e), necrotic cells (N), fibrillary collagen (C) and abundant ground substance (G) are seen. C: The corneoscleral trabecular meshwork is constituted by a quite disintegrated tissue. The trabecular beams (TB) (double arrows) have an “empty” appearance and are separated by intertrabecular spaces filled with debris of necrotic endothelial cells (N) and detached collagen (C) from the trabecular beams. D: The image shows fused trabecular beams (TB) (double arrows) and in some zones between them, debris from necrotic cells (white arrowhead) appears. The trabecular beams is constituted by a basal membrane (bm), many elastic-like fibers (e), fibrillary collagen (C), and abundant ground substance (G). E: The image shows two endothelial cells, one shows necrosis (N) and the other one has autophagic activity (arrow). F and G: Detail of the autophagic activity of an endothelial trabecular cell. F: Mitophagy (mp). G: Autophagosome (ap) with a double-membrane containing cytoplasmic material and organelles: mitochondria (M), rough endoplasmic reticulum (RER) and ribosomes (R).