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. 2017 Apr 27;12(4):e0176550. doi: 10.1371/journal.pone.0176550

Fig 2. Effect of pH and temperature on the catalytic activity of AfmE1.

Fig 2

(A) Determination of optimum pH. The hydrolytic activity was measured at different pHs at 40°C for 10 min. (B) Determination of optimum temperature. The hydrolytic activity was measured at temperatures ranging from 20 to 70°C. (C) Thermal stability assay. The enzyme was incubated at 45, 50 and 55°C for up to 4 h and residual activity was determined under the optimal reaction conditions. Error bars represent the standard deviation.