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. 2017 Apr 27;12(4):e0176639. doi: 10.1371/journal.pone.0176639

Fig 1. Corticosteroids effects on keratocytes under normal culture conditions.

Fig 1

(A)Morphological results of keratocytes after 5-d treatments with PBS, PA, Dex and Flu. (B)Proliferation in each group was determined by CCK8 assayed at 12 h, and then every 24 h during 5 days. (C)Short-term cell viabilities in each group were tested by CCK8 at 12 h (a) and 24 h (b). (D)The migration rates of keratocytes at 12 h(a) and 24 h(b) were evaluated by Image Pro-Plus software (Media Cybernetics) using the following formula: (0 h distance—set time distance)/ time (Pixel/h). (E)Migration abilities of keratocytes grown in normal condition with corticosteroid treatments at 0 h, 12 h and 24 h. Data were analyzed by one way ANOVA,*p<0.05 versus control group (n = 3).