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. 2017 Apr 17;13(4):e1006328. doi: 10.1371/journal.ppat.1006328

Fig 7. GPATCH3 interrupts assembly of the VISA-associated signaling complexes.

Fig 7

(A) 293T cells (2 x 106) were cotransfected with Flag-VISA (3 μg) and HA-tagged TBK1, TRAF6 or TRAF3 (3 μg), together with increasing amounts of GPATCH3 (0, 0.5 μg, 1.5μg). Coimmunoprecipitation and immunoblotting were performed with the indicated antibodies. (B) Wild-type or GPACH3-deficient cells (1.5 x 107) were left uninfected or infected with SeV for the indicated times. Coimmunoprecipitation and immunoblotting were performed with the indicated antibodies.