Figure 2.

Effect of 17β‐E2, DPI, AEBSF, Mito Tempo and NAC on intracellular ROS and apoptosis in MLO‐Y4 cells. Intracellular ROS (A) and apoptosis (B) were measured in MLO‐Y4 cells cultured for 4 and 24 h in complete medium (C, control) or in serum‐free medium (S, starved cells). S were treated or not with 10 nm 17β‐E2, 1 μm DPI, 100 μm AEBSF, 5 nm Mito‐TEMPO, or 5 mm NAC as reported in Materials and methods. ROS data, normalized on total protein content, and apoptosis data, relative to mono‐ and oligonucleosomes released into the cytoplasmic fraction from 10⁴ cells, are expressed as fold‐increase over the respective C values. The data are the mean ± SEM of four independent experiments. *P ≤ 0.05 and **P ≤ 0.005 compared to the respective S values.